Tunicamycin是一种抗生素，可通过竞争性抑制UDP-GlcNAc-dolichol-phosphate N-acetylglucosamine-1 phosphate transferase (DPAGT1)活性来有效抑制N-连接糖基化(N-linked glycosylation, NLG)，并可通过减少血管生成、抑制菌落来靶向多种类型的肿瘤形成，并增强TRAIL诱导的细胞凋亡。
| CAS No. | 11089-65-9 |
| 生物活性 | Tunicamycin is a mixture of homologous nucleoside antibiotic that inhibits N-linked glycosylation and blocks GlcNAc phosphotransferase (GPT). Tunicamycin causes accumulation of unfolded proteins in cell endoplasmic reticulum (ER) and induces ER stress, and causes blocking of DNA synthesis and cell cycle arrest in G1 phase. Tunicamycin inhibits gram-positive bacteria, yeasts, fungi, and viruses and has anti-cancer activity.Tunicamycin increases exosome release in cervical cancer cells. |
| 分子式 | C30H46N4O16 |
| 分子量 | 844.94 (n=10) |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存条件 | Powder, -20°C ,3 years |
| 溶解性数据 | DMSO : 33.33 mg/mL (Need ultrasonic)，H2O : 10 mg/mL (adjust pH to 12 with 1 M NaOH) |
| 体内研究 | Tunicamycin (0.1 mg/kg or 0.5 mg/kg) treatment dramatically suppresses tumor growth in the CD133 MHCC97L cells xenograft model (BALB/c (nu/nu) mice). |
| 体外研究 | Tunicamycin (2 µg/mL; 24 hours; CD44+/CD24- and original MCF7 cells) treatment increases the spliced XBP-1, ATF6 nuclear translocation level and CHOP protein expression in CD44+/CD24- and original MCF7 cells.Tunicamycin-induced ER stress suppresses CD44+/CD24- phenotype cell subpopulation and in vitro invasion and accelerates tumorosphore formation. Under effect of Tunicamycin, the results show that inhibited invasion, increased cell death, suppressed proliferation and reduced migration in the CD44+/CD24- and CD44+/CD24- rich MCF7 cell culture. |
| 纯度及产品资料 | 98% |
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