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抑制剂/激活剂

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SAG

SAG is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG activates the Hedgehog signaling pathway and counteracts Cyclopamine inhibition of Smo.

货号：
TK0482
规格：

1mg

2mg

5mg

10mg
价格：
￥0.00

产品参数

CAS No.	912545-86-9
生物活性	SAG is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG activates the Hedgehog signaling pathway and counteracts Cyclopamine inhibition of Smo.
分子式	C28H28ClN3Os
分子量	490.06
运输条件	Room temperature in continental US; may vary elsewhere.
储存条件	4°C, protect from light
溶解性数据	DMSO : ≥ 38 mg/mL (77.54 mM) H2O : < 0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble)
体内研究	SAG (1.0 mM) induces more osteogenesis mainly at the defect borders and a significant increase in BV/TV at the eight week timepoint in CD-1 mice. SAG (15-20 mg/kg; i.p.) induces pre-axial polydactyly prevalently in a dose-dependent manner in mice.
体外研究	SAG (0.1 nM-100 μM; 30 h) induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM and then inhibits expression at higher concentrations. SAG (1-1000 nM; 1 h) competes for the binding of BODIPY-cyclopamine to Smo-expressing Cos-1 cells, yielding an apparent dissociation constant (Kd) of 59 nM for the SAG/Smo complex. SAG (100 nM) inhibits the inhibitory effect of ShhN-induced pathway activation by Robotnikinin. SAG (250 nM; 48 h) significantly increases SMO mRNA and protein expression in MDAMB231 cells. SAG (250 nM; 24 and 48 h) increases CAXII mRNA expression in MDAMB231 cells at 24h in normoxic and hypoxic conditions in MDAMB231 cells. SAG (250 nM; 24 h) increases MDAMB231 cells migration.
文献	•Cell Rep. 2020 Apr. •Glia. 2021 Mar 11. •Development. 2018 Sep 20;145(18). pii: dev164830. •Cancer Sci. 2021 Oct;112(10):4176-4186. •Biochem Pharmacol. 2021 May 6;190:114593. •Toxins. 2021 Aug 22;13(8):585. •Molecules. 2017 Dec 31;23(1). pii: E85. •Drug Dev Res. 2019 Nov;80(7):992-999. •Mol Brain. 2021 Jul 19;14(1):116. •J Pharm Biomed Anal. 2017 Aug 5;142:201-209. •Research Square Preprint. 2021 Sep. •Rheinische Friedrich-Wilhelms-Universität Bonn. Mathematisch-Naturwissenschaftlichen Fakultät. 2021 May 21.
纯度及产品资料	98%

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