H2DCFDA (DCFH-DA)是一种可渗透细胞的探针，可用于检测细胞内 reactive oxygen species (ROS) 的产生。它在细胞内去酯化，并在氧化后转化为高荧光的 2',7'-Dichlorodihydrofluorescein。 它用于对氧化代谢反应中的氧反应物质进行灵敏、快速的定量，检测吞噬细胞中氧化产物的微孔板测定，以及定量多孔骨髓分化测定。(Ex/Em=488/525 nm)
| CAS No. | 4091-99-0 |
| 生物活性 | H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm). |
| 分子式 | H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm). |
| 分子量 | 487.29 |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存条件 | (-20°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| 溶解性数据 | DMSO : 125 mg/mL (256.52 mM; Need ultrasonic) Ethanol : 20 mg/mL (41.04 mM; Need ultrasonic) |
| 体外研究 | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use. 2. Cells are incubated with 5 µM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. 3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA. |
| 纯度及产品资料 | 98% |
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